Direct Detection of Transcription Factors in Cotyledons during Seedling Development Using Sensitive Silicon-Substrate Photonic Crystal Protein Arrays

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  • Additional Information
    • Publication Information:
      American Society of Plant Biologists, 2015.
    • Publication Date:
      2015
    • Abstract:
      Transcription factors control important gene networks, altering the expression of a wide variety of genes, including those of agronomic importance, despite often being expressed at low levels. Detecting transcription factor proteins is difficult, because current high-throughput methods may not be sensitive enough. One-dimensional, silicon-substrate photonic crystal (PC) arrays provide an alternative substrate for printing multiplexed protein microarrays that have greater sensitivity through an increased signal-to-noise ratio of the fluorescent signal compared with performing the same assay upon a traditional aminosilanized glass surface. As a model system to test proof of concept of the silicon-substrate PC arrays to directly detect rare proteins in crude plant extracts, we selected representatives of four different transcription factor families (zinc finger GATA, basic helix-loop-helix, BTF3/NAC [for basic transcription factor of the NAC family], and YABBY) that have increasing transcript levels during the stages of seedling cotyledon development. Antibodies to synthetic peptides representing the transcription factors were printed on both glass slides and silicon-substrate PC slides along with antibodies to abundant cotyledon proteins, seed lectin, and Kunitz trypsin inhibitor. The silicon-substrate PC arrays proved more sensitive than those performed on glass slides, detecting rare proteins that were below background on the glass slides. The zinc finger transcription factor was detected on the PC arrays in crude extracts of all stages of the seedling cotyledons, whereas YABBY seemed to be at the lower limit of their sensitivity. Interestingly, the basic helix-loop-helix and NAC proteins showed developmental profiles consistent with their transcript patterns, indicating proof of concept for detecting these low-abundance proteins in crude extracts.
    • Author Affiliations:
      Departments of Crop Sciences (S.I.J., M.S., L.V.) and Electrical and Computer Engineering (Y.T., S.G., B.T.C.), University of Illinois at Urbana-Champaign, Urbana, Illinois 61801
    • ISSN:
      00320889
      15322548
    • Rights:
      Copyright © 2015 American Society of Plant Biologists
    • Accession Number:
      edsjsr.24801778
  • Citations
    • ABNT:
      JONES SARAH I. et al. Direct Detection of Transcription Factors in Cotyledons during Seedling Development Using Sensitive Silicon-Substrate Photonic Crystal Protein Arrays. Plant Physiology, [s. l.], v. 167, n. 3, p. 639, 2015. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.24801778&custid=s6224580. Acesso em: 26 fev. 2020.
    • AMA:
      Jones Sarah I., Tan Yafang, Shamimuzzaman Md, George Sherine, Cunningham Brian T., Vodkin Lila. Direct Detection of Transcription Factors in Cotyledons during Seedling Development Using Sensitive Silicon-Substrate Photonic Crystal Protein Arrays. Plant Physiology. 2015;167(3):639. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.24801778&custid=s6224580. Accessed February 26, 2020.
    • APA:
      Jones Sarah I., Tan Yafang, Shamimuzzaman Md, George Sherine, Cunningham Brian T., & Vodkin Lila. (2015). Direct Detection of Transcription Factors in Cotyledons during Seedling Development Using Sensitive Silicon-Substrate Photonic Crystal Protein Arrays. Plant Physiology, 167(3), 639.
    • Chicago/Turabian: Author-Date:
      Jones Sarah I., Tan Yafang, Shamimuzzaman Md, George Sherine, Cunningham Brian T., and Vodkin Lila. 2015. “Direct Detection of Transcription Factors in Cotyledons during Seedling Development Using Sensitive Silicon-Substrate Photonic Crystal Protein Arrays.” Plant Physiology 167 (3): 639. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.24801778&custid=s6224580.
    • Harvard:
      Jones Sarah I. et al. (2015) ‘Direct Detection of Transcription Factors in Cotyledons during Seedling Development Using Sensitive Silicon-Substrate Photonic Crystal Protein Arrays’, Plant Physiology, 167(3), p. 639. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.24801778&custid=s6224580 (Accessed: 26 February 2020).
    • Harvard: Australian:
      Jones Sarah I., Tan Yafang, Shamimuzzaman Md, George Sherine, Cunningham Brian T. & Vodkin Lila 2015, ‘Direct Detection of Transcription Factors in Cotyledons during Seedling Development Using Sensitive Silicon-Substrate Photonic Crystal Protein Arrays’, Plant Physiology, vol. 167, no. 3, p. 639, viewed 26 February 2020, .
    • MLA:
      Jones Sarah I., et al. “Direct Detection of Transcription Factors in Cotyledons during Seedling Development Using Sensitive Silicon-Substrate Photonic Crystal Protein Arrays.” Plant Physiology, vol. 167, no. 3, 2015, p. 639. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.24801778&custid=s6224580.
    • Chicago/Turabian: Humanities:
      Jones Sarah I., Tan Yafang, Shamimuzzaman Md, George Sherine, Cunningham Brian T., and Vodkin Lila. “Direct Detection of Transcription Factors in Cotyledons during Seedling Development Using Sensitive Silicon-Substrate Photonic Crystal Protein Arrays.” Plant Physiology 167, no. 3 (2015): 639. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.24801778&custid=s6224580.
    • Vancouver/ICMJE:
      Jones Sarah I., Tan Yafang, Shamimuzzaman Md, George Sherine, Cunningham Brian T., Vodkin Lila. Direct Detection of Transcription Factors in Cotyledons during Seedling Development Using Sensitive Silicon-Substrate Photonic Crystal Protein Arrays. Plant Physiology [Internet]. 2015 [cited 2020 Feb 26];167(3):639. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.24801778&custid=s6224580