Recognition of conserved antigens by Th17 cells provides broad protection against pulmonary Haemophilus influenzae infection.

Item request has been placed! ×
Item request cannot be made. ×
  Processing Request
  • Additional Information
    • Publication Year:
      2018
    • Author-Supplied Keywords:
      antigenic diversity
      Haemophilus influenzae
      pneumonia
      Th17 responses
      vaccine
    • NAICS/Industry Codes:
      NAICS/Industry Codes 325410 Pharmaceutical and medicine manufacturing
      325414 Biological Product (except Diagnostic) Manufacturing
    • Abstract:
      Nontypeable Haemophilus influenzae (NTHi) is a major cause of community acquired pneumonia and exacerbation of chronic obstructive pulmonary disease. A current effort in NTHi vaccine development has focused on generating humoral responses and has been greatly impeded by antigenic variation among the numerous circulating NTHi strains. In this study, we showed that pulmonary immunization of mice with killed NTHi generated broad protection against lung infection by different strains. While passive transfer of immune antibodies protected only against the homologous strain, transfer of immune T cells conferred protection against both homologous and heterologous strains. Further characterization revealed a strong Th17 response that was cross-reactive with different NTHi strains. Responding Th17 cells recognized both cytosolic and membrane-associated antigens, while immune antibodies preferentially responded to surface antigens and were highly strain specific. We further identified several conserved proteins recognized by lung Th17 cells during NTHi infection. Two proteins yielding the strongest responses were tested as vaccine candidates by immunization of mice with purified proteins plus an adjuvant. Immunization induced antigen-specific Th17 cells that recognized different strains and, upon adoptive transfer, conferred protection. Furthermore, immunized mice were protected against challenge with not only NTHi strains but also a fully virulent, encapsulated strain. Together, these results show that the immune mechanism of cross-protection against pneumonia involves Th17 cells, which respond to a broad spectrum of antigens, including those that are highly conserved among NTHi strains. These mechanistic insights suggest that inclusion of Th17 antigens in subunit vaccines offers the advantage of inducing broad protection and complements the current antibody-based approaches. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • ISSN:
      00278424
    • Accession Number:
      131086575
  • Citations
    • ABNT:
      WENCHAO LI et al. Recognition of conserved antigens by Th17 cells provides broad protection against pulmonary Haemophilus influenzae infection. Proceedings of the National Academy of Sciences of the United States of America, [s. l.], v. 115, n. 30, p. E7149, 2018. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131086575&custid=s6224580. Acesso em: 26 jan. 2020.
    • AMA:
      Wenchao Li, Xinyun Zhang, Ying Yang, et al. Recognition of conserved antigens by Th17 cells provides broad protection against pulmonary Haemophilus influenzae infection. Proceedings of the National Academy of Sciences of the United States of America. 2018;115(30):E7149. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131086575&custid=s6224580. Accessed January 26, 2020.
    • APA:
      Wenchao Li, Xinyun Zhang, Ying Yang, Qingqin Yin, Yan Wang, Yong Li, … Hao Shen. (2018). Recognition of conserved antigens by Th17 cells provides broad protection against pulmonary Haemophilus influenzae infection. Proceedings of the National Academy of Sciences of the United States of America, 115(30), E7149. Retrieved from http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131086575&custid=s6224580
    • Chicago/Turabian: Author-Date:
      Wenchao Li, Xinyun Zhang, Ying Yang, Qingqin Yin, Yan Wang, Yong Li, Chuan Wang, et al. 2018. “Recognition of Conserved Antigens by Th17 Cells Provides Broad Protection against Pulmonary Haemophilus Influenzae Infection.” Proceedings of the National Academy of Sciences of the United States of America 115 (30): E7149. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131086575&custid=s6224580.
    • Harvard:
      Wenchao Li et al. (2018) ‘Recognition of conserved antigens by Th17 cells provides broad protection against pulmonary Haemophilus influenzae infection’, Proceedings of the National Academy of Sciences of the United States of America, 115(30), p. E7149. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131086575&custid=s6224580 (Accessed: 26 January 2020).
    • Harvard: Australian:
      Wenchao Li, Xinyun Zhang, Ying Yang, Qingqin Yin, Yan Wang, Yong Li, Chuan Wang, Wong, SM, Ying Wang, Goldfine, H, Akerley, BJ & Hao Shen 2018, ‘Recognition of conserved antigens by Th17 cells provides broad protection against pulmonary Haemophilus influenzae infection’, Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 30, p. E7149, viewed 26 January 2020, .
    • MLA:
      Wenchao Li, et al. “Recognition of Conserved Antigens by Th17 Cells Provides Broad Protection against Pulmonary Haemophilus Influenzae Infection.” Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 30, July 2018, p. E7149. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131086575&custid=s6224580.
    • Chicago/Turabian: Humanities:
      Wenchao Li, Xinyun Zhang, Ying Yang, Qingqin Yin, Yan Wang, Yong Li, Chuan Wang, et al. “Recognition of Conserved Antigens by Th17 Cells Provides Broad Protection against Pulmonary Haemophilus Influenzae Infection.” Proceedings of the National Academy of Sciences of the United States of America 115, no. 30 (July 24, 2018): E7149. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131086575&custid=s6224580.
    • Vancouver/ICMJE:
      Wenchao Li, Xinyun Zhang, Ying Yang, Qingqin Yin, Yan Wang, Yong Li, et al. Recognition of conserved antigens by Th17 cells provides broad protection against pulmonary Haemophilus influenzae infection. Proceedings of the National Academy of Sciences of the United States of America [Internet]. 2018 Jul 24 [cited 2020 Jan 26];115(30):E7149. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=131086575&custid=s6224580