The Groucho-related Gene Family Regulates the Gonadotropin-releasing Hormone Gene through Interaction with the Homeodomain Proteins MSX1 and OCT1.

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  • Source:
    Journal of biological chemistry, 2005 Sep. 2, v. 280, no. 35, p. 30975-30983. 280 35
  • Language:
    English
  • Publication Type:
    Journal Article
  • Online Access:
    http://www.jbc.org/
  • Publication Date:
    Sep 2, 2005
  • Additional Information
    • Author:
      Rave-Harel, Naama; Mellon, Pamela L.; Givens, Marjory L.; Miller, Nichol L. G.
    • Abstract:
      Gonadotropin-releasing hormone (GnRH) is exclusively expressed in a unique population of hypothalamic neurons that controls reproductive function. GnRH gene expression is highly dynamic. Its transcriptional activity is regulated in a complex spatiotemporal manner during embryonic development and postnatal life. Although a variety of transcription factors have been identified as regulators of GnRH transcription, most are promiscuous in their DNA-binding requirements, and none are solely expressed in GnRH neurons. Their specific activity is probably determined by interactions with distinct cofactors. Here we find that the Groucho-related gene (GRG) family of co-repressors is expressed in a model cell line for the GnRH neuron and co-expresses with GnRH during prenatal development. GRG proteins associate in vivo with the GnRH promoter. Furthermore, GRG proteins interact with two regulators of GnRH transcription, the homeodomain proteins MSX1 and OCT1. Co-transfection experiments indicate that GRG proteins regulate GnRH promoter activity. The long GRG forms enhance MSX1 repression and counteract OCT1 activation of the GnRH gene. In contrast, the short form, GRG5, has a dominant-negative effect on MSX1-dependent repression. Taken together, these data suggest that the dynamic switch between activation and repression of GnRH transcription is mediated by recruitment of the GRG co-regulators.
    • Subject Terms:
      Internet resource
    • Notes:
      Includes references.
    • ISSN:
      00219258
    • Call Numbers:
      NAL Call Number. 381 J824
    • Subset:
      Indexing Branch - NAL
    • Publication Date:
      00000000
    • Accession Number:
      IND43738875
  • Citations
    • ABNT:
      RAVE-HAREL, N. et al. The Groucho-related Gene Family Regulates the Gonadotropin-releasing Hormone Gene through Interaction with the Homeodomain Proteins MSX1 and OCT1. Journal of biological chemistry, [s. l.], v. 280, n. 35, p. 30975–30983, 2005. DOI http://www.jbc.org/. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=agr&AN=IND43738875&custid=s6224580. Acesso em: 12 dez. 2019.
    • AMA:
      Rave-Harel N, Mellon PL, Givens ML, Miller NLG. The Groucho-related Gene Family Regulates the Gonadotropin-releasing Hormone Gene through Interaction with the Homeodomain Proteins MSX1 and OCT1. Journal of biological chemistry. 2005;280(35):30975-30983. doi:http://www.jbc.org/.
    • APA:
      Rave-Harel, N., Mellon, P. L., Givens, M. L., & Miller, N. L. G. (2005). The Groucho-related Gene Family Regulates the Gonadotropin-releasing Hormone Gene through Interaction with the Homeodomain Proteins MSX1 and OCT1. Journal of Biological Chemistry, 280(35), 30975–30983. https://doi.org/http://www.jbc.org/
    • Chicago/Turabian: Author-Date:
      Rave-Harel, Naama, Pamela L. Mellon, Marjory L. Givens, and Nichol L. G. Miller. 2005. “The Groucho-Related Gene Family Regulates the Gonadotropin-Releasing Hormone Gene through Interaction with the Homeodomain Proteins MSX1 and OCT1.” Journal of Biological Chemistry 280 (35): 30975–83. doi:http://www.jbc.org/.
    • Harvard:
      Rave-Harel, N. et al. (2005) ‘The Groucho-related Gene Family Regulates the Gonadotropin-releasing Hormone Gene through Interaction with the Homeodomain Proteins MSX1 and OCT1’, Journal of biological chemistry, 280(35), pp. 30975–30983. doi: http://www.jbc.org/.
    • Harvard: Australian:
      Rave-Harel, N, Mellon, PL, Givens, ML & Miller, NLG 2005, ‘The Groucho-related Gene Family Regulates the Gonadotropin-releasing Hormone Gene through Interaction with the Homeodomain Proteins MSX1 and OCT1’, Journal of biological chemistry, vol. 280, no. 35, pp. 30975–30983, viewed 12 December 2019, .
    • MLA:
      Rave-Harel, Naama, et al. “The Groucho-Related Gene Family Regulates the Gonadotropin-Releasing Hormone Gene through Interaction with the Homeodomain Proteins MSX1 and OCT1.” Journal of Biological Chemistry, vol. 280, no. 35, Sept. 2005, pp. 30975–30983. EBSCOhost, doi:http://www.jbc.org/.
    • Chicago/Turabian: Humanities:
      Rave-Harel, Naama, Pamela L. Mellon, Marjory L. Givens, and Nichol L. G. Miller. “The Groucho-Related Gene Family Regulates the Gonadotropin-Releasing Hormone Gene through Interaction with the Homeodomain Proteins MSX1 and OCT1.” Journal of Biological Chemistry 280, no. 35 (September 2, 2005): 30975–83. doi:http://www.jbc.org/.
    • Vancouver/ICMJE:
      Rave-Harel N, Mellon PL, Givens ML, Miller NLG. The Groucho-related Gene Family Regulates the Gonadotropin-releasing Hormone Gene through Interaction with the Homeodomain Proteins MSX1 and OCT1. Journal of biological chemistry [Internet]. 2005 Sep 2 [cited 2019 Dec 12];280(35):30975–83. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=agr&AN=IND43738875&custid=s6224580